Primary cultures of prostate epithelial cells and their ability to activate carcinogens.

Differences in the incidence of prostate cancer (CaP) amongst different migrant populations point to causative agents of dietary and/or environmental origin. Prostate tissues were obtained following transurethral resection of the prostate (TURP) or radical retropubic prostatectomy. After surgery, TURP-derived or tumour-adjacent tissue fragments were minced in warm PFMR-4A medium (37?C) and suspensions pipetted into collagen-coated petri dishes. Non-adherent material was removed by washing with fresh medium after 12 h. Adhered cells subsequently reacted positively with monoclonal antibodies to prostate specific antigen (PSA). PSA was also detected in the medium. The genotoxicities of the chemical carcinogens 2-amino-1-methyl-6-phenylimidazo4,5-b pyridine (PhIP), its N-hydroxy metabolite (N-OH-PhIP) and benzoapyrene (BaP) in adherent cell populations from different donors (n=8) were examined. Cells were treated in suspension for 30 min at 37?C in the presence of the DNA repair inhibitors hydroxyurea (HU) and cytosine arabinoside (ara-C). DNA single-strand breaks were detected in cells by the alkaline single cell-gel electrophoresis ('Comet') assay and quantified by measuring comet tail length (CTL) in m. All three carcinogens induced dose-related increases in CTLs (P{\ensuremath{<}}0.0001) in cells from four donors 24 h post-seeding. However, in cells from a further two donors the genotoxic effects of PhIP, N-OH-PhIP and BaP were much less apparent after 48 h than after 24 h in culture. After 96 h in culture, cells from these donors appeared to be resistant to the comet-forming activity of the compounds. However, BaP-DNA adducts were still measurable by 32P-postlabelling for up to 14 days following a 24-h exposure to 50 M BaP in adhered cells from another two donors. This study shows that primary cultures of cells derived from the prostate can activate members of two classes of chemical carcinogens. Further development may provide a robust model system in which to investigate the aetiology of CaP

Supporting Enhancement of Stewardship in Small-Scale Fisheries: Perceptions of Governance Among Caribbean Coral Reef Fishers

Small-scale fishing livelihoods dependent on Caribbean coral reefs face an uncertain future with global climate change and mounting anthropogenic pressures threatening ecosystem integrity and resilience. In the context of future threats to coral reefs, improved governance is critical to enhance the efficacy of coral reef management. Recent research places increasing emphasis on identifying governance arrangements that enable participation and engagement, with the improved ‘social fit’ of institutions expected to engender stewardship among fishers. However, few studies have examined the perspectives of resource users in relation to a wide range of articulated principles for good governance processes. This study contributes to an improved understanding of how fisher perceptions relate to diverse governance arrangements in the Wider Caribbean Region. We quantify perceptions among 498 reef-dependent fishers in relation to principles of ‘good governance’ in 12 communities across four Caribbean countries: Barbados, Belize, Honduras, and St. Kitts and Nevis. We describe perceptions relating to two underlying governance themes – institutional acceptance (reflecting principles of legitimacy, transparency, fairness, and connectivity) and engagement in reef governance (reflecting principles of accountability and inclusiveness). In addition, we identify socio-demographic factors associated with each set of perceptions and explore the implications for future governance of small-scale Caribbean reef fisheries. The findings suggest that an understanding of heterogeneous perceptions within small-scale fisheries can inform more targeted interventions to improve the fit of governance arrangements for different groups. Governance may be more effective if perceptions are used to identify areas in which to pursue greater engagement of resource users in stewardship